How to extract highly free DNA from plasma (New Product Promotion)
As we all know, We first need to efficiently isolate free DNA from plasma. This is not as simple as imagined because the amount of free DNA in plasma is very low, usually less than 10 ng/mL., Cells in the human body can occasionally undergo apoptosis. During this process, DNA is fragmented and secreted into the extracellular domain. Healthy cells, tumor cells, fetal cells and transplanted cells release DNA into the blood. With this free nucleic acid analysis, we can noninvasively monitor disease, detect aneuploidy in unborn babies, or understand graft rejection.
Therefore, free nucleic acid has become a hot topic in medical research today. In cancer research, DNA can use for monitoring disease progression, treatment, or cancer recurrence. In addition, free nucleic acid analysis can often lead to earlier diagnosis than invasive methods. This analysis is called the hot fluid biopsy nowadays.
In the process of liquid biopsy, people usually use EDTA to collect blood vessels or specialized blood vessels. After the cell components were precipitated in the blood, the supernatant was used to separate the free DNA (cfDNA). Then, cfDNA was quantified by qPCR or capillary electrophoresis. Subsequently, sensitive methods such as digital PCR, quantitative PCR or NGS can be used to detect microRNAs, single nucleotide mutations or chromosomal mutations.
Of course, we first need to efficiently isolate free DNA from plasma. This is not as simple as imagined because the amount of free DNA in plasma is very low, usually less than 10 ng/mL. Fortunately, there are special kits in the market. For example, the SANLI MEDICAL CELL-FREE DNA COLLECTION TUBES.
The tubes uses silica gel technology to efficiently extract free DNA from 1-10 mL plasma. The NucleoSnap DNA Plasma Column in the tube can handle up to 5 mL of plasma and is eluted in very small volumes (20-50 mL), bringing highly concentrated DNA.
The whole operation process is also familiar with washing —Elution. First, the plasma sample was mixed with protease K and binding buffer, and the sample was added to silica gel column. After that, vacuum filtration was used to combine DNA with silica gel mold. Two step washing can effectively remove pollutants, such as PCR inhibitors. Finally, the highly purified DNA was eluted by elution buffer. The whole process takes about 45 minutes.
As for the production of free DNA, it depends on donor health, blood collection and processing, plasma preparation and other factors. A large proportion of free DNA originates from apoptotic cells, so they may be highly fragmented. NucleoSnap DNA Plasma kit can separate 50-1000 BP fragments of DNA, so you don’t miss any key information.
If there are more samples in the lab, Will you choose the high quality SANLI MEDICAL CELL-FREE DNA COLLECTION TUBES? It looks good. Let’s have a try.Tags: Cell-Free DNA Collection Tubes, cfDNA, DNA